IL-2: IL-2RA Inhibitor Screening Colorimetric Assay Kit | 78584

IL-2: IL-2RA Inhibitor Screening Colorimetric Assay Kit | 78584 | Gentaur US, UK & Europe Disrtribition

Category: Cytokine/Assay Kit

Application: Screen inhibitors of IL-2 binding to IL-2RA.

Background: Interleukin-2 (IL-2) is a cytokine secreted by activated T lymphocytes to stimulate the proliferation of both B and T cells. IL-2 is involved in the extreme inflammation response termed "cytokine storm" observed following viral infection in susceptible patients or following adaptive cell therapy. Thus, it is considered a therapeutic target for the treatment of severe COVID-19. IL-2 acts by binding to a membrane heterotrimeric receptor consisting of IL-2 receptor alpha (IL2RA, also known as CD25) and beta (IL2RB) chains, which together with the common gamma chain (IL2RG) form a high-affinity receptor, whereas IL-2RA homodimers form a low-affinity receptor. Polymorphisms in the genes coding for IL2RA and IL2RB may be associated with risk of lung cancer.

Description: The IL-2: IL-2 Receptor Alpha (IL-2RA) Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of inhibitors or neutralizing antibodies blocking the interaction between human IL-2 and its receptor IL-2RA.  The assay requires only a few steps. First, IL-2RA is coated on a 96-well plate overnight. After blocking, the receptor is pre-incubated with the test inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-IL-2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

Product Type: Assay Kit

Shippement Condition: -80°C