Description
IDO1 Cellular Activity QuickDetect™ Supplements | 62000-1 | Gentaur US, UK & Europe Disrtribition
Category: Immunotherapy/Cell Line
Application: Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context
Background: L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. IDO1 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description: The IDO1 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1- expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of tryptophan 2,3 dioxygenase (IDO1)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Product Type: Media & Reagents
Shippement Condition: gel packs (-20°C)
