Description
ICAM1 Antibody / CD54 | V2604IHC-7ML | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Format: Purified
Clone: 1H4 or W-CAM-1 or Wehi-CAM-1
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: IHC-P
Buffer: N/A
Limitation: This ICAM-1 antibody is available for research use only.
Purity: Protein G affinity chromatography
Description: Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 mAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This mAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Immunogen: Raji Burkitt lymphoma cells were used as the immunogen for the ICAM-1 antibody.
Storage: Store the ICAM-1 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
