ICAM-3 Antibody / CD50 | V2607-100UG

ICAM-3 Antibody / CD50 | V2607-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: 101-1D2

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: FACS, IF, IHC-P

Buffer: N/A

Limitation: This ICAM-3 antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.

Immunogen: Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

Storage: Store the ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).