Description
Human VEGF165-coupled Magnetic Beads | MBS-K036-10mg| Gentaur Distribution US, UK & Europe
VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
Source: Human VEGF165 Protein is expressed from human 293 cells (HEK293) . It contains AA Ala 27 - Arg 191 (Accession # P15692-4) .
Molecule: VEGF165.
Format: Powder.
Species: Human.
Application: This product is intended for immunocapture, biopanning and flow cytometry. This is a non-sterile product.
Kit Component: Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Stability: Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C. Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Storage Conditions: -20℃, aviod light.
Shipping Conditions: RT
