Human UCH-L1 Protein, His Tag | UC1-H5140-1mg

Human UCH-L1 Protein, His Tag | UC1-H5140-1mg| Gentaur Distribution US, UK & Europe

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also, UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.

Source: Human UCH-L1, His Tag (UC1-H5140) is expressed from E. coli cells. It contains AA Gln 2 - Ala 223 (Accession # NP_004172).

Format: Powder.

Tag: N-6×His.

Expression System: E. coli.

Expression Region: Gln 2 - Ala 223.

Conjugate: Unconjugated.

Molecular Weight: 25.7 kDa.

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 27-28 kDa under reducing (R) condition (SDS-PAGE).

Purity: 95%.

Buffer: 50 mM Tris, 150 mM Nacl, pH8.0.

Storage Conditions: -20℃.

Shipping Conditions: RT