Description
Human SIRT1(Sirtuin 1) CLIA Kit | E-CL-H0992 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 3.5h
Detection Type: Chemiluminescence
Sensitivitys: 37.5pg/mL
Detection Range: 62.5~4000pg/mL
Target Name: SIRT1
Target Synonyms: SIR2L1, NAD-dependent deacetylase sirtuin-1
Tested Sample: Serum, plasma and other biological fluids
Test Principale: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the RLU value of the samples to the standard curve.
Specificity: This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed.
Application: This CLIA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in Serum, plasma and other biological fluids.
Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Tags and Cell Markers
Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
