Human Siglec-7 / CD328 Protein, Fc (L118A, G120A, E201A) Tag (MALS verified) | SI7-H5257-50ug

Human Siglec-7 / CD328 Protein, Fc (L118A, G120A, E201A) Tag (MALS verified) | SI7-H5257-50ug| Gentaur Distribution US, UK & Europe

Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

Source: Human Siglec-7, Fc (L118A, G120A, E201A) Tag (SI7-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Format: Powder.

Tag: C-Fc (A3).

Expression System: HEK293.

Expression Region: Gln 19 - Leu 353.

Conjugate: Unconjugated.

Molecular Weight: 63.3 kDa.

Characteristics: This protein carries a Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.

Purity: 95%.

Buffer: 25 mM MES, 150 mM NaCl.

Storage Conditions: -20℃.

Shipping Conditions: RT