Description
Human Siglec-3 / CD33 (R119G) Protein, Fc Tag | CD3-H5254-1mg| Gentaur Distribution US, UK & Europe
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
Source: Human Siglec-3 (R119G), Fc Tag (CD3-H5254) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1 (R119G) ).
Format: Powder.
Tag: C-Fc.
Expression System: HEK293.
Expression Region: Asp 18 - His 259.
Conjugate: Unconjugated.
Molecular Weight: 53.1 kDa.
Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 64-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
