Human SGPP1(Sphingosine-1-Phosphate Phosphatase 1) ELISA Kit | E-EL-H2581

Human SGPP1(Sphingosine-1-Phosphate Phosphatase 1) ELISA Kit | E-EL-H2581 | Gentaur US, UK & Europe Disrtribition

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 37.5pg/mL

Detection Range: 62.5~4000pg/mL

Target Name: SGPP1

Target Synonyms: SPPase1

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SGPP1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SGPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SGPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human SGPP1. You can calculate the concentration of Human SGPP1 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human SGPP1 in samples. No significant cross-reactivity or interference between Human SGPP1 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human SGPP1 concentrations in Serum, plasma and other biological fluids.

Research Area: Cell Biology

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.