Human PH20 / SPAM1 Protein, Tag Free (active enzyme, MALS verified) | PH0-H5219-25ug

Human PH20 / SPAM1 Protein, Tag Free (active enzyme, MALS verified) | PH0-H5219-25ug| Gentaur Distribution US, UK & Europe

Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4) -linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Source: Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).

Format: Powder.

Tag: Native.

Expression System: HEK293.

Expression Region: Leu 36 - Ser 490.

Conjugate: Unconjugated.

Molecular Weight: 52.6 kDa.

Characteristics: This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: 50 mM Tris, 100 mM NaCl, pH7.5.

Storage Conditions: -20℃.

Shipping Conditions: RT