Human PH20 / SPAM1 Protein, His Tag (active enzyme) DMF Filed | PH0-H5225-200ug

Human PH20 / SPAM1 Protein, His Tag (active enzyme) DMF Filed | PH0-H5225-200ug| Gentaur Distribution US, UK & Europe

Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4) -linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Source: Human PH20, His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).

Format: Powder.

Tag: C-10×His.

Expression System: HEK293.

Expression Region: Leu 36 - Tyr 482.

Conjugate: Unconjugated.

Molecular Weight: 53.0 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: 50 mM Tris, 100 mM NaCl, pH7.5.

Storage Conditions: -20℃.

Shipping Conditions: RT