Human Interleukin-12 | 90178-B

Human Interleukin-12 | 90178-B | Gentaur US, UK & Europe Disrtribition

Category: Cytokine

Application: N/A

Background: IL-12 is secreted by peripheral lymphocytes after induction. It is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL-12 are bacteria, bacterial products, and parasites. IL-12 is produced after stimulation with phorbol esters or calcium ionophore by human B-lymphoblastoid cells. The IL-12 receptor, CD212, appears to be a single protein of approximately 110 kDa. Up to 1000-9000 high affinity IL-12 receptors/cell are expressed on peripheral blood mononuclear cells activated by various mitogens for T-cells or by IL2. IL-12 receptors are present on activated T-cells expressing CD4 and CD8 and on activated CD56(+) natural killer cells. Resting peripheral blood mononuclear cells, tonsillar B-cells, or tonsillar B-cells activated by anti-IgM/Dx, anti-IgM/Dx + IL-2, or SAC + IL-2 do not express the receptor. Human IL-12 is not active in murine lymphocytes. Hybrid heterodimers consisting of murine p35 and human p40 subunits retain bioactivity on murine cells, however, the combination of human p35 and murine p40 is completely inactive on murine cells. Murine IL-12 is active on both murine and human lymphocytes.

Description: Recombinant Interleukin-12 is a disulfide-linked heterpdimer protein consisting of 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit, and migrates due to glycosylation as an approximately 75 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-12 mature chain was expressed in Chinese Hamster Ovary (CHO) cells.

Product Type: Protein

Shippement Condition: gel packs or dry ice