Description
Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (MALS verified) | IN4-H52W3-100ug| Gentaur Distribution US, UK & Europe
Human integrin alpha (10) I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha (10) I did recognize collagen types I-VI and laminin-1 in a Mg (2+) -dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.
Source: Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (IN4-H52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 | Asn 28 - Ser 710 (Accession # P23229-2 & P16144-2).
Format: Powder.
Tag: C-10×His | Native.
Expression System: HEK293.
Expression Region: Phe 24 - Gly 1012 | Asn 28 - Ser 710.
Conjugate: Unconjugated.
Molecular Weight: 116.8 kDa | 81.8 kDa.
Characteristics: Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) respectively due to glycosylation.
Purity: 95%.
Buffer: 50 mM Tris, 150 mM NaCl, 0.2 M Arginine, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
