Description
Human IL-8 ELISA Kit | CRS-B004| Gentaur Distribution US, UK & Europe
ClinMaxTM ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
Source: ClinMaxTM ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
Molecule: ILN/A8.
Format: Solid.
Species: Human.
Application: The kit is developed for quantitative detection of IL-8 in human serum and cell culture supernates.
Kit Component: This assay kit is used to measure the concentrations of human Interleukin 8 (IL-8) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-8 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-8 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Stability: The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Storage Conditions: 2-8℃.
Shipping Conditions: RT
