Human IL-8 | 90258-A

Human IL-8 | 90258-A | Gentaur US, UK & Europe Disrtribition

Category: Cytokine

Application: N/A

Background: IL-8 is produced by stimulated monocytes but not by tissue macrophages or T-lymphocytes. In many cell types the synthesis of IL-8 is strongly stimulated by IL-1 and TNF-alpha. In human skin fibroblasts, the expression of IL-8 is enhanced by Leukoregulin.The expression of IL-8 from resting and stimulated human blood monocytes is up-regulated by IL-7. In chondrocytes, the synthesis of IL-8 is stimulated by IL1-beta TNF-alpha and bacterial lipopolysaccharides. In human astrocytes, the synthesis and secretion of IL-8 is induced by IL-1 and TNF-alpha. The IL-8 receptor (CD128) is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It is expressed in many different cell types including those not responding to IL-8. The biological activities of IL-8 resemble those of a related protein, NAP-2. IL-8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. In neutrophils, IL-8 causes a transient increase in cytosolic calcium levels and the release of enzymes from granules. IL-8 also enhances the metabolism of reactive oxygen species and increases chemotaxis and the enhanced expression of adhesion molecules. Pre-activation by IL-3 is required to render basophils and neutrophils susceptible to further activation by IL-8. Native human IL-8, generated by the proteolytic removal of the signal peptide and propeptide, has a calculated molecular mass of approximately 8 kDa.

Description: Recombinant human Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residues, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in E. coli.

Product Type: Protein

Shippement Condition: gel packs or dry ice