Description
Human CD40 / TNFRSF5 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) | CD0-H525a-1mg| Gentaur Distribution US, UK & Europe
CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Source: Human CD40 Protein, Mouse IgG2a Fc Tag (CD0-H525a) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).
Format: Powder.
Tag: C-mFc (mIgG2a).
Expression System: HEK293.
Expression Region: Glu 21 - Arg 193.
Conjugate: Unconjugated.
Molecular Weight: 46.1 kDa.
Characteristics: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.1 kDa. The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
