Description
Human CD33-coupled Magnetic Beads | MBC-K008-10mg| Gentaur Distribution US, UK & Europe
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
Source: Human CD33 protein is expressed from human 293 cells (HEK293) . It contains AA Asp 18 - His 259 (Accession # P20138-1 (R69G) ) .
Molecule: Siglec-3.
Format: Powder.
Species: Human.
Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
Kit Component: Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Stability: Upon receipt, please store the beads at -20°C. The shelf life is 1 year at -20 °C. Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Storage Conditions: -20℃, aviod light.
Shipping Conditions: RT
