Description
Human AP36(Apelin 36) ELISA Kit | E-EL-H0459 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Competitive
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 28.13pg/mL
Detection Range: 46.88~3000pg/mL
Target Name: AP36
Target Synonyms: AP36
Tested Sample: Serum, plasma and other biological fluids
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human AP36. During the reaction, Human AP36 in samples or Standard competes with a fixed amount of Human AP36 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human AP36. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human AP36 in samples. No significant cross-reactivity or interference between Human AP36 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human AP36 concentrations in Serum, plasma and other biological fluids.
Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction
Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
