Description
Hs 578T Cell Line | CL-0114 | Gentaur US, UK & Europe Disrtribition
Media: CM-0114
Organism: Homo sapiens, Human
Application: N/A
Background: The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning. Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy. As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected.
Age: 74 years adult
Tissue: Mammary gland/breast
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+0.01mg/ml Insulin+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: No, in immunosuppressed mice; Yes, in semisolid medium.
Duration: N/A
Lead Time: 3-5 days
