Description
HMy2.CIR cell line | CL-0112 | Gentaur US, UK & Europe Disrtribition
Media: CM-0112
Organism: Homo sapiens, Human
Application: N/A
Background: This cell line is established by gamma irradiation and selection for loss of HLA class I antigen expression from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line. The cells can be utilized as host for transfected class I major histocompatibility antigen genes. The ARH-77 cell line was reported to be positive for Epstein-Barr nuclear antigen (EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). Therefore, the HMy2.CIR cell line is assumed to be EBNA positive. The cells express small amounts of HLA Cw4 but no HLA A or B locus products.
Age: 33 years
Tissue: B-Lymphoblast
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 2
Medium: IMDM(PM150510)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 2×10^5 and 1×10^6 viable cells/ml.
Ratio: 2×10^5 to 1×10^6 cells/mL
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
