Description
HLA-DRB1 Antibody (MHC II) | V2591-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: L243
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: FACS, IF
Buffer: N/A
Limitation: This HLA-DRB1 antibody is available for research use only.
Purity: Protein G affinity chromatography
Description: This mAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the alpha/beta heterodimer. This mAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Immunogen: Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
Storage: Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
