HL-60 Cell Line | CL-0110

HL-60 Cell Line | CL-0110 | Gentaur US, UK & Europe Disrtribition

Media: CM-0110

Organism: Homo sapiens, Human

Application: Transfection.

Background: HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.

Age: 36 years

Tissue: Peripheral blood

Morphology: Myeloblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: IMDM(PM150510)+20% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice(subcutaneous myeloid tumors). Yes, in semi-solid media.

Duration: N/A

Lead Time: 3-5 days