Histone H2AX Antibody (pS139) | RQ4503

Histone H2AX Antibody (pS139) | RQ4503 | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol

Format: Purified

Clone: AbH41

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG

Species Reactivity: Human, Mouse

Application: IHC-P, IF/ICC, WB

Application Details: Western blot: 1:500-1:1000ug/ml,IHC (FFPE): 1:50-1:200,IF/ICC: 1:50-1:200

Application Note: Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.

Purity: Protein A affinity

Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]

Immunogen: The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.

Storage: After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Localization: Nucleus