Description
His Antibody | F52059-0.2ML | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azide
Format: Purified
Clone: 6AT18
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: N/A
Application: WB, ELISA
Buffer: N/A
Limitation: This anti-His antibody is available for research use only.
Purity: Purified
Description: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Immunogen: Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody.
Storage: Store at 4oC for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.