Description
HIF1 bata Monoclonal Antibody | E-AB-22189 | Gentaur US, UK & Europe Disrtribition
Reactivity: Mouse
Host: Mouse
Isotype: IgG
Application: WB, IHC-p, IF
Research Area: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).
Lead Time: 7~10 days
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Recombinant Protein
Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Protein A purification
Dilution: WB 1:1000-2000, IHC 1:100-200
Conjugation: Unconjugated
Calculated Molecular Weight: N/A
Observed Molecular Weight: 87 kDa
Clonality: Monoclonal
