Description
Hela S3 cell line | CL-0350 | Gentaur US, UK & Europe Disrtribition
Media: CM-0350
Organism: Homo sapiens, human
Application: N/A
Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura.
Age: Female,31 years
Tissue: Cervix
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~48 hours
Biosafty: 2
Medium: Ham's F-12K(PM150910)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
