Hela S3 cell line | CL-0350

Hela S3 cell line | CL-0350 | Gentaur US, UK & Europe Disrtribition

Media: CM-0350

Organism: Homo sapiens, human

Application: N/A

Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura.

Age: Female,31 years

Tissue: Cervix

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~48 hours

Biosafty: 2

Medium: Ham's F-12K(PM150910)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:5

Renewal: Every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days