Description
HEC-1-B Cell Line | CL-0100 | Gentaur US, UK & Europe Disrtribition
Media: CM-0100
Organism: Homo sapiens, Human
Application: Transfection host.
Background: This is a substrain of HEC-1-A. Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th and 190th days in culture and appeared on recovery to be flattened and more pavement patterned than the parent line.The predominant complement of chromosomes was double that observed for the parent line.
Age: 71 years
Tissue: Uterus, endometrium
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in nude mice. The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma(gradeⅡ). Yes, in steroid-treated hamsters.
Duration: N/A
Lead Time: 3-5 days
