Description
HCoV-229E Spike protein, His Tag | SPN-H52H3-500ug| Gentaur Distribution US, UK & Europe
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source: HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 1115 (Accession # P15423-1 (TI 871-872 PP) ).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.
Format: Powder.
Tag: C-10×His.
Expression System: HEK293.
Expression Region: Cys 16 - Pro 1115.
Conjugate: Unconjugated.
Molecular Weight: 125.7 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 90%.
Buffer: PBS.
Storage Conditions: -20℃.
Shipping Conditions: RT
