Description
HAL-01 cell line | CL-0588 | Gentaur US, UK & Europe Disrtribition
Media: CM-0588
Organism: Homo sapiens, Human
Application: N/A
Background: This cell line was established in 1990 from the peripheral blood of a 17-year-old female with acute lymphoblastic leukemia (ALL-L2). The cell line contains the fusion gene of t(17; 19)(q22; p13) TCF3-HLF (E2A-HLF).
Age: female, 17 years
Tissue: B Cell Precursor Leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: ~48 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
Ratio: 1:2 to 1:3
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days