H9c2(2-1) Cell Line | CL-0089

H9c2(2-1) Cell Line | CL-0089 | Gentaur US, UK & Europe Disrtribition

Media: CM-0089

Organism: Rattus norvegicus, Rat

Application: Transfection host.

Background: Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.Fusion occurs faster if the serum concentration in the medium is reduced to one percent.

Age: Embryo

Tissue: Heart/myocardium

Morphology: Myoblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days