Description
GFAP Antibody / Glial Fibrillary Acidic Protein | V3589SAF-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free
Format: Purified
Clone: rASTRO/789
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human, Rat
Application: FACS, WB, IHC-P
Application Details: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note: The optimal dilution of the recombinant Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein G affinity chromatography
Description: This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this recombinant Glial Fibrillary Acidic Protein antibody.
Storage: Store the recombinant Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic
