GENPower™ NLS-Cas12a Nuclease (MALS verified) | CAA-L5140-100ug

GENPower™ NLS-Cas12a Nuclease (MALS verified) | CAA-L5140-100ug| Gentaur Distribution US, UK & Europe

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) -associated endonuclease from Lachnospiraceae bacterium，Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system, an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/RNA (crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.

Source: GENPower™ NLS-Cas12a Nuclease (CAA-L5140) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

Format: Liquid.

Tag: N-10×His.

Expression System: E. coli.

Expression Region: Ser 2 - His 1228.

Conjugate: Unconjugated.

Molecular Weight: 147.6 kDa.

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.

Purity: 95%.

Buffer: 20 mM NaAC, 500 mM NaCl, 0.1 mM EDTA, 0.1 mM TCEP, pH 6.0.

Storage Conditions: -20℃.

Shipping Conditions: Dry ice