Description
GC-1 spg Cell Line | CL-0600 | Gentaur US, UK & Europe Disrtribition
Media: CM-0600
Organism: Mus Musculus, Mouse, transgenic
Application: N/A
Background: Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance)
Age: 10 days
Tissue: Testis
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2
Medium: DMEM (PM150210)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: 1:4 to 1:6
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+59% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days