Description
GAS Reporter (Luc) - HeLa Cell Line (IFNγ/JAK/STAT1 Pathway) | 79041 | Gentaur US, UK & Europe Disrtribition
Category: Cell Line/Cell Signaling Pathway
Application: • Monitor interferon gamma-induced signal transduction pathways. • Screen for activators or inhibitors of JAK/STAT1 signaling pathway using the GAS Reporter Cell Line.
Background: N/A
Description: The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.
Product Type: Cell Line
Shippement Condition: -80°C
