Description
GAPDH Polyclonal Antibody | E-AB-40337 | Gentaur US, UK & Europe Disrtribition
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Application: WB, IHC
Research Area: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Lead Time: 7~10 days
Concentration: 0.66 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Purification: Antigen Affinity Purification
Dilution: WB 1:3000-1:6000 IHC 1:300-1:500
Conjugation: Unconjugated
Calculated Molecular Weight: 35 kDa
Observed Molecular Weight: 35 kDa
Clonality: Polyclonal
