Description
GAPDH Antibody | RQ4477 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol
Format: HRP Conjugate
Clone: A-7
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human, Mouse, Rat
Application: WB
Application Details: Western blot: 1:5000-1:20000
Application Note: Optimal dilution of the GAPDH antibody should be determined by the researcher.
Purity: Protein A affinity
Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt]
Immunogen: A synthetic peptide from human GAPDH was used as the immunogen for the GAPDH antibody.
Storage: Store the GAPDH antibody at -20 °C.
Localization: Cytoplasm, Nucleus, Membrane
