Description
FTO Chemiluminescent Assay Kit | 79344 | Gentaur US, UK & Europe Disrtribition
Category: Demethylase/Assay Kit
Application: Great for studying enzyme kinetics and HTS applications.
Background: The methylation of internal adenosines at the N6 position (m6A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer's disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m6A demethylase identified.
Description: The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Product Type: Assay Kit
Shippement Condition: -80°C
