Description
FTH Antibody / Ferritin heavy chain | V8509-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: FTH/2082
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: WB, IHC-P
Buffer: N/A
Limitation: This FTH antibody is available for research use only.
Purity: Protein G affinity chromatography
Description: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.
Immunogen: A portion of amino acids 58-180 from the human protein was used as the immunogen for the FTH antibody.
Storage: Store the FTH antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
