Description
Fluorescent Human Claudin-6 / CLDN6 Protein, Flag, His, GFP Tag (Nanodisc) | CL6-HF2M9-20ug| Gentaur Distribution US, UK & Europe
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Claudin-6, also knows as CLDN6, is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. And it is one of the entry cofactors for hepatitis C virus. The methylation of CLDN6 may be involved in esophageal tumorigenesis. The gene of CLDN6 is adjacent to another family member CLDN9 on chromosome 16.
Source: Fluorescent Human Claudin-6 Protein, Flag, His, GFP Tag (CL6-HF2M9) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).
Format: Liquid.
Tag: N-Flag & N-10×His & C-GFP.
Expression System: HEK293.
Expression Region: Ala 2 - Val 220.
Conjugate: GFP Fusion.
Molecular Weight: 54.0 kDa.
Characteristics: This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus. The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 80%.
Buffer: 20 mM HEPES, 150 mM NaCl, pH7.5.
Storage Conditions: -70℃.
Shipping Conditions: Dry ice
