Description
FGL1:LAG3 TR-FRET Assay Kit | 79739-2 | Gentaur US, UK & Europe Disrtribition
Category: Immunotherapy/Assay Kit
Application: This kit is useful for screening for inhibitors of LAG3 binding to FGL1.
Background: Lymphocyte-activation gene 3 (LAG3, also CD223) is a cell surface receptor that negatively regulates activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.
Description: The FGL1:LAG3 TR-FRET Assay is designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated LAG3, His-tagged FGL1 protein, and an inhibitor are incubated for one hour. Then, anti-His Tb donor and dye-labeled acceptor are added and fluorescence intensity is measured using a fluorescence reader.
Product Type: Assay Kit
Shippement Condition: -80°C
![LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit](https://cdn11.bigcommerce.com/s-63go3c7jq9/images/stencil/590x590/products/118171/131399/BPS__94768.1677778340.jpg?c=1 "LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit")
