FGL1:LAG3 TR-FRET Assay Kit | 79739-1

FGL1:LAG3 TR-FRET Assay Kit | 79739-1 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Assay Kit

Application: This kit is useful for screening for inhibitors of LAG3 binding to FGL1.

Background: Lymphocyte-activation gene 3 (LAG3, also CD223) is a cell surface receptor that negatively regulates activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.

Description: The FGL1:LAG3 TR-FRET Assay is designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated LAG3, His-tagged FGL1 protein, and an inhibitor are incubated for one hour. Then, anti-His Tb donor and dye-labeled acceptor are added and fluorescence intensity is measured using a fluorescence reader.

Product Type: Assay Kit

Shippement Condition: -80°C