F9 Cell Line | CL-0082

F9 Cell Line | CL-0082 | Gentaur US, UK & Europe Disrtribition

Media: CM-0082

Organism: Mus musculus, Mouse

Application: Transfection host.

Background: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP(cAMP).Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.cAMP is active only on cells that have been treated with retinoic acid.The cells maintain three copies of the beta 1 integrin gene.Tested and found negative for ectromelia virus(mousepox).

Age: Embryo

Tissue: Testis

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days