Description
Estrogen Receptor alpha Antibody | V3687-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: NR3Ga-4R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: IHC-P, WB
Application Details: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note: The stated application concentrations are suggested starting points. Titration of the recombinant Estrogen Receptor alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.
Immunogen: Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor alpha antibody.
Storage: Store the recombinant Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Nuclear
