Estrogen Receptor alpha Antibody | V3581-20UG

Estrogen Receptor alpha Antibody | V3581-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: ESR1/2299R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: IHC-P, WB, FACS

Application Details: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Application Note: The optimal dilution of the recombinant Estrogen Receptor antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Immunogen: Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor antibody.

Storage: Store the recombinant Estrogen Receptor antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Nuclear