EpCAM Antibody | V8936-100UG

EpCAM Antibody | V8936-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: rEGP40/826

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml

Application Note: Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Purity: Protein A/G affinity

Description: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Immunogen: Recombinant human Ep-CAM protein was used as the immunogen for the recombinant EpCAM antibody. The epitope is known to be between amino acids 27-59.

Storage: Aliquot the recombinant EpCAM antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.

Localization: Cell surface, cytoplasmic