Description
EpCAM Antibody | V8649-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: EGP40/4546R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note: Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
Purity: Protein A affinity chromatography
Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Immunogen: Recombinant full-length human EpCAM protein was used as the immunogen for the recombinant EpCAM antibody. The binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.
Storage: Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface, cytoplasmic
