EpCAM Antibody | V7264-20UG

EpCAM Antibody | V7264-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: EGP40/1555R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: FACS, IHC-P, IF

Application Details: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Immunogen: Recombinant full length protein was used as the immunogen for the recombinant EpCAM antibody.

Storage: Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface, cytoplasmic