EpCAM Antibody | V3465SAF-100UG

EpCAM Antibody | V3465SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: rVU-1D9

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P, FACS

Application Details: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells

Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Ep-CAM antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein G affinity chromatography

Description: Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. The VU-1D9 antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Immunogen: Small cell lung carcinoma cells were used as the immunogen for this recombinant Ep-CAM antibody.

Storage: Store the recombinant Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface, cytoplasmic