Description
EpCAM Antibody | V3464SAF-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free
Format: Purified
Clone: rMOC-31
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: IHC-P, IF, FACS, WB
Application Details: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Application Note: Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein G affinity chromatography
Description: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for the recombinant EpCAM antibody. Binding epitope of the rMOC-31 antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.
Storage: Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface, cytoplasmic
